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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
30/06/2021 |
Actualizado : |
30/06/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASTRO JANER, E.; DÍAZ, A.; FONTES, F.; BARAIBAR, F.; SAPORITI, T.; OLHAGARAY, M. E. |
Afiliación : |
ELEONOR CASTRO JANER, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; ANDRÉ DÍAZ, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA FONTES, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA BARAIBAR, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; TATIANA SAPORITI NOGUEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA ERNESTINA OLHAGARAY, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay. |
Título : |
Molecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Ticks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747 |
ISSN : |
1877-959X |
DOI : |
10.1016/j.ttbdis.2021.101747 |
Idioma : |
Inglés |
Notas : |
Article history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021.
The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35).
Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com |
Contenido : |
ABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021 MenosABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals an... Presentar Todo |
Palabras claves : |
Acaricides; Ixodidae; Reaxys retrieves substances; Rhipicephalus microplus. |
Asunto categoría : |
L73 Enfermedades de los animales |
Marc : |
LEADER 03773naa a2200265 a 4500 001 1062207 005 2021-06-30 008 2021 bl uuuu u00u1 u #d 022 $a1877-959X 024 7 $a10.1016/j.ttbdis.2021.101747$2DOI 100 1 $aCASTRO JANER, E. 245 $aMolecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay.$h[electronic resource] 260 $c2021 500 $aArticle history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021. The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35). Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com 520 $aABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021 653 $aAcaricides 653 $aIxodidae 653 $aReaxys retrieves substances 653 $aRhipicephalus microplus 700 1 $aDÍAZ, A. 700 1 $aFONTES, F. 700 1 $aBARAIBAR, F. 700 1 $aSAPORITI, T. 700 1 $aOLHAGARAY, M. E. 773 $tTicks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
21/02/2014 |
Actualizado : |
10/07/2017 |
Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
Autor : |
BALMELLI, G.; RESQUÍN, F.; SIMETO, S.; TORRES, D.; NUÑEZ, P.; RODRIGUEZ, F.; GONZALEZ, C. |
Afiliación : |
GUSTAVO DANIEL BALMELLI HERNANDEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; JOSE FERNANDO RESQUIN PEREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFIA SIMETO FERRARI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; DIEGO GABRIEL TORRES DINI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PABLO TABARE NUÑEZ RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FEDERICO GONZALO RODRIGUEZ ALBORNOZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; CARLOS WILFREDO GONZALEZ BENAVIDEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Mancha amarilla en eucaliptos colorados: importancia de la especie y fuente de semilla utilizada |
Fecha de publicación : |
2014 |
Fuente / Imprenta : |
Revista INIA Uruguay, 2014, no. 36, p. 36-40 |
Serie : |
(Revista INIA; 36) |
ISSN : |
1510-9011 |
Idioma : |
Español |
Palabras claves : |
EUCALIPTOS COLORADOS. |
Thesagro : |
ENFERMEDADES DE LAS PLANTAS; EUCALIPTOS; EUCALYPTUS CAMALDULENSIS; EUCALYPTUS TERETICORNIS; FORESTACION. |
Asunto categoría : |
K10 Producción forestal |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3260/1/Revista-INIA-36-p.-36.pdf
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Marc : |
LEADER 00793naa a2200277 a 4500 001 1028255 005 2017-07-10 008 2014 bl uuuu u00u1 u #d 022 $a1510-9011 100 1 $aBALMELLI, G. 245 $aMancha amarilla en eucaliptos colorados$bimportancia de la especie y fuente de semilla utilizada 260 $c2014 490 $a(Revista INIA; 36) 650 $aENFERMEDADES DE LAS PLANTAS 650 $aEUCALIPTOS 650 $aEUCALYPTUS CAMALDULENSIS 650 $aEUCALYPTUS TERETICORNIS 650 $aFORESTACION 653 $aEUCALIPTOS COLORADOS 700 1 $aRESQUÍN, F. 700 1 $aSIMETO, S. 700 1 $aTORRES, D. 700 1 $aNUÑEZ, P. 700 1 $aRODRIGUEZ, F. 700 1 $aGONZALEZ, C. 773 $tRevista INIA Uruguay, 2014, no. 36, p. 36-40
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